Members of Nidogen proteins are multivalent matrix binding proteins that function as classical linkers joining laminin and collagen IV networks in basement membranes. Lack of nidogens completely prevented BM deposition and ultrastructural assembly of BM and hemidesmosomes, although other BM proteins remained detectable at comparable levels with no signs of degradation. Supplementation by recombinant nidogen restored these structures. Nidogen-1 (NID-1) is also known as Entactin. Entactin is an integral and ubiquitous component of basement membrane (BM) alongside other components such as collagen type IV, proteoglycans (heparan sulfate and glycosaminoglycans), laminin and fibronectin in the skin epidermal-dermal junction. The protein interacts with several other components of basement membranes and is essential for connecting laminins to collagen IV in the basement membrane. It may also play a role in cell interactions with the extracellular matrix. Entactin binds calcium ions and this calcium-binding activity may play a role in the matrix assembly process.

Recent scientific investigations have identified Nidogens as key components in the aging process. Discoveries show their importance as support connectors for skin firmness. After 3 years of research, L’Bel and Sederma Laboratories have created Nidogen-X Complexes Jour: the topical treatment whose technology replenishes Nidogens or “Vital Connectors” that tend to erode as the skin ages, providing the skin with improved firmness, reduction of wrinkles and deep hydration.